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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which ends up in chromosome instability, and thus may perhaps add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant function in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, happen to be explained. Two added splice variants ended up documented but detailed nucleotide sequence details is not really nevertheless accessible.
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Blocking peptides are peptides that bind exclusively into the goal antibody and block antibody binding. These peptide ordinarily consists of the epitope identified by the antibody.
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This manual outlines additional probable optimization of mobile and immunoassay parameters to make sure the best possible success are...
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This guideline outlines further possible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind towards the epitope around the target protein. By comparing the staining from the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Focus on Details Cyclin E belongs for the extremely conserved cyclin family members, whose users are characterized by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins functionality as regulators of CDK kinases. Various cyclins show distinct expression and degradation styles which lead on the temporal coordination of each mitotic function. Cyclin E types a complex with and capabilities like a regulatory more info subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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